Science
Mechanism of Action
Ozonized Olive Oil (OOO) exerts its action through the interaction of ozone-derived compounds, primarily peroxides, aldehydes, and ozonides, with cellular components. It functions as a bio-regulator, re-establishing cellular redox balance and modulating cytokine levels like interleukin-8, TNF-alpha, TGF-beta, and PDGF, which are crucial for immune response and tissue remodeling. OOO's active compounds stimulate fibroblast proliferation, leading to increased production of structural proteins like collagen and elastin for tissue repair and scar reduction. Its germicidal effects against bacteria and fungi stem from the oxidation of microbial cell walls. Furthermore, the slow release of oxygen from breaking down ozonides activates keratinoblast proliferation and supports the building of the intercellular matrix, promoting holistic healing while combating inflammation and purifying the treated area.
Research
Clinical Evidence
High confidence2%
Key findings
- 01 Demonstrated broad-spectrum antimicrobial activity at 2% concentration against S. aureus, E. coli, P. aeruginosa, B. subtilis, and C. albicans.
- 02 Exhibited half-maximal inhibitory concentrations (IC50) as low as 0.03% against Candida albicans and 0.04% against Enterococcus faecalis.
- 03 Effective against Staphylococcus aureus at 0.08% IC50, and against Escherichia coli and Pseudomonas aeruginosa at 1.25% IC50.
Transparency
Dusting Analysis
Ozonized Olive Oil is an active ingredient with well-documented therapeutic applications across a wide range of dermatological conditions, suggesting it is intentionally included for its benefits rather than as a 'dusting' ingredient.
The Formula
Formulation
Stability
Ozonized olive oil exhibits good shelf life, maintaining therapeutic potential for up to a year at room temperature and three to four years when refrigerated. Refrigeration or freezing is recommended for extended stability, as ambient or elevated temperatures can lead to rapid chemical degradation. Products should be stored in dark glass bottles to protect against sunlight. Ozonation increases the oil's density and viscosity, which are favorable characteristics for cosmetic formulations. The acid value (AV) serves as a key quality parameter.
Safety
Safety Profile
Preliminary evidence indicates ozonated liquids are well tolerated and present no significant dermatological risks. Ozonated olive oil is considered suitable for all skin types, including sensitive skin, demonstrating very good skin compatibility in dermatological tests. While generally safe, mild cytotoxicity has been observed in normal cell lines (LLC-PK1 and HaCaT) at concentrations above 0.0625% (625 µg/mL), though clinical efficacy is noted at higher percentages (up to 2-5%) without widespread reports of adverse dermatological reactions in human applications.
Your Skin
Skin Compatibility
Our Assessment
Verdict
Ozonized Olive Oil is a valuable, multi-functional ingredient, leveraging enhanced antimicrobial activity and significant regenerative properties to support skin healing and combat various dermatological concerns, suitable for all skin types.
Related
Similar Ingredients
Finding similar ingredients…
References
Sources