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Moumoujus

ENDONUCLEASE V.

Valuable / SKIN PROTECTING

Endonuclease V is an advanced enzyme specifically designed to repair DNA damage caused by ultraviolet (UV) radiation, particularly cyclobutane pyrimidine dimers (CPDs). When encapsulated in liposomes, it effectively penetrates the skin to enhance natural repair processes, offering a proactive approach to combating photoaging and promoting skin regeneration.

Summary

DNA Repair Anti-aging Photoprotection Skin Regeneration UV Damage Mitigation

Science

Mechanism of Action

Endonuclease V (T4N5) precisely identifies and binds to UV-induced cyclobutane pyrimidine dimers (CPDs) within cellular DNA. This initiates a critical DNA repair pathway by creating a single-strand break at the dimer site, facilitating the removal and replacement of damaged DNA segments. Delivered via pH-sensitive liposomes, this 16 kDa hydrophilic protein effectively permeates the stratum corneum to reach epidermal keratinocytes and Langerhans cells. Its action can amplify natural DNA repair by up to fourfold, mitigating photoaging by reducing MMP-1 induction and preventing the breakdown of extracellular matrix components. The enzyme also recognizes other forms of DNA damage, including deaminated bases, abasic sites, and structural anomalies.

Research

Clinical Evidence

High confidence
Effective range N/A
Optimal

N/A

Key findings

  1. 01 Topical application of liposome-encapsulated T4 endonuclease V reduced the incidence of basal cell carcinomas by 30% and actinic keratoses by over 68% in patients with xeroderma pigmentosum over one year.
  2. 02 In a study of 12 xeroderma pigmentosum patients, topical application of liposomal T4 endonuclease V to UV-exposed skin led to a 20% reduction in cyclobutane pyrimidine dimers (CPDs) in skin biopsies within 6 hours.

Transparency

Dusting Analysis

Not commonly dusted

Endonuclease V is a high-value active ingredient requiring specialized liposomal encapsulation for effective skin penetration and stability. Its complex delivery system and specific enzymatic mechanism make it unsuitable for use as a 'dusted' ingredient, indicating that effective formulations would likely contain meaningful concentrations.

The Formula

Formulation

Solubility
Water
Optimal pH 1.5 – 5.5
0 7 14

Stability

As a 16 kDa hydrophilic protein, T4 endonuclease V requires pH-sensitive liposomal encapsulation for enhanced stability, efficient cellular delivery into keratinocytes, and optimal skin permeation. It is sensitive to heat, with inactivation occurring at 65°C after 20 minutes.

Conflicts

  • strong acids
  • strong bases
  • high temperatures (above 65°C)
  • certain solvents
  • heavy metals
  • strong oxidizing agents
  • strong reducing agents

Safety

Safety Profile

CIR Status
Not reviewed
Sensitization risk Low

Clinical trials involving topical liposome-encapsulated T4 endonuclease V demonstrated minimal adverse effects and no evidence of allergic or irritant contact dermatitis. Animal testing also confirmed effective repair without skin irritation. The ingredient 'T4 endonuclease V (T4N5)' has received an INCI name and a PCPC file number (25213) under the trademark LipoT4N5.

Your Skin

Skin Compatibility

Yes Normal
Yes Dry
Yes Oily
Yes Sensitive
Irritancy Low
Comedogenicity Unknown

Our Assessment

Verdict

Valuable

Endonuclease V is a valuable ingredient offering precise DNA repair capabilities to protect against and reverse UV-induced damage, making it a critical component for advanced anti-photoaging and skin regeneration formulations.

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