Science
Mechanism of Action
This synthetic tripeptide functions as a neurotransmitter inhibitor by acting as an antagonist to the muscular nicotinic acetylcholine receptor (mnAChR). By blocking acetylcholine binding, it impedes nerve impulse transmission to facial muscles, inducing a relaxed state. This action consequently reduces the frequency and intensity of muscle contractions, effectively diminishing expression lines and wrinkles. Beyond muscle relaxation, it can also stimulate collagen and elastin production, leading to improved skin texture.
Research
Clinical Evidence
High confidence4%
Key findings
- 01 An in-vivo study spanning 28 days, utilizing a 4% concentration, demonstrated an average of 21% smoother skin and a 15-20% reduction in wrinkle depth, with some participants achieving up to a 52% reduction. These smoothing effects were measurable on 80% of volunteers, and wrinkle reduction on 73%.
- 02 Another in-vivo test reported an impressive reduction of over 50% in wrinkle size after 28 days, though the precise concentration was not specified.
- 03 Recommended use levels for achieving significant activity typically fall within a 1-4% concentration of the solution in the final formulation.
Transparency
Dusting Analysis
While clinical data supports significant activity at concentrations between 1-4% (of the solution), reported supplier usage levels can be as low as 0.005%. This considerable difference between the recommended efficacious range and the lowest noted usage suggests a potential for 'dusting,' where the ingredient may be included at sub-therapeutic levels primarily for marketing claims rather than delivering its full functional benefits.
The Formula
Formulation
Stability
The ingredient is chemically stable, with its diacetate groups enhancing solubility and stability in aqueous solutions. For product integrity, it should be stored protected from direct light and humidity, ideally between 10°C-25°C. For long-term storage, the powder is best kept at 2-8°C, or at -20°C for up to 1 year. Stock solutions maintain stability for up to 24 months at 4°C. It should be incorporated into formulations during the final manufacturing stage, ensuring temperatures remain below 40°C. Encapsulation techniques, such as liposomes and nanoparticles, can further augment its stability and delivery.
Conflicts
- May interact with certain preservatives or emulsifiers, potentially diminishing its effectiveness or increasing the risk of irritation.
Safety
Safety Profile
Dipeptide Diaminobutyroyl Benzylamide Diacetate Oligopeptide-133 is widely considered safe for use in anti-aging skincare products. The Environmental Working Group (EWG) rates it as 1 out of 10, indicating a low hazard level. While PubChem lists a GHS classification of 'Toxic in contact with skin' for the raw, concentrated substance, this is typically not relevant for its application in diluted cosmetic formulations. Some individuals may rarely experience mild skin irritation (e.g., redness, itching, burning) or, in rare cases, allergic reactions like hives or swelling.
Your Skin
Skin Compatibility
Our Assessment
Verdict
A highly effective biomimetic peptide that provides significant reduction in expression lines and wrinkles through targeted muscle relaxation, making it a valuable asset for precision anti-aging formulations.
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