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ACETYL HEXAPEPTIDE-24 AMIDE.

Questionable CAS 616204-22-9 / SKIN CONDITIONING

A precision peptide, Acetyl Hexapeptide-24 Amide works to visibly diminish the appearance of dynamic wrinkles and fine lines. It achieves this through a dual mechanism, moderating facial muscle contractions while simultaneously stimulating the skin's natural collagen and hyaluronic acid production for a firmer, more hydrated complexion.

humectant skin conditioning anti-wrinkle collagen stimulating skin firming

Science

This hexapeptide, also known as Acetyl Hexapeptide-8 Amide, actively targets the neuromuscular junction by mimicking a fragment of the SNAP-25 protein. This interference disrupts the SNARE complex, which is crucial for the release of neurotransmitters that initiate muscle contractions. By impeding this signaling, the peptide promotes facial muscle relaxation, leading to a reduction in the depth and visibility of expression wrinkles. Furthermore, it enhances skin's structural integrity by stimulating collagen synthesis and boosts hydration and plumpness through increased hyaluronic acid production.


Research

High confidence
Effective range 0.05–10%
Optimal

0.05-0.2%

Key findings

  1. 01 Demonstrated significant improvements in both wrinkle depth and number over a four-week period in a study involving 31 subjects.
  2. 02 Reduced wrinkle area by an average of 11.3% after only 5 days in a study of female volunteers.
  3. 03 Achieved an average decrease of 12.3% in wrinkle depth after 5 days, observed in a study of female volunteers.
  4. 04 Yielded nearly a 30% improvement in periorbital rhytids (wrinkles around the eyes) after 30 days in a cohort of 10 healthy women.

Transparency

Commonly dusted
Min. effective
0.005%
Red flag below
0.005%

Clinical evidence supports the efficacy of Acetyl Hexapeptide-24 Amide at concentrations ranging from 0.05% to 10%. However, the Cosmetic Ingredient Review (CIR) Expert Panel concluded that it is safe for leave-on products only up to 0.005%, citing insufficient data to determine safety at higher concentrations. This significant disparity between proven effective doses and regulatory-supported safe concentrations strongly suggests that products formulated within CIR guidelines may contain 'dusting' levels, rendering them unlikely to deliver the advertised benefits.


The Formula

Solubility
Water
Optimal pH 4 – 7
0 7 14

Stability

The peptide maintains optimal stability within a pH range of 4.0 to 7.0. Its inherent hydrophilic nature and relatively large molecular size can pose challenges for skin penetration. Effective delivery can be enhanced through sophisticated formulation strategies such as oil-in-water (O/W) and multiple water-in-oil-in-water (W/O/W) emulsions.


Safety

CIR Status
Safe with restrictions
Max tested
0.005%
Sensitization risk Low

The CIR Expert Panel deemed Acetyl Hexapeptide-8 Amide (a synonym) safe for use in leave-on cosmetic products at concentrations up to 0.005%. Data are currently insufficient to ascertain its safety at concentrations exceeding this threshold. The FDA's stance on ingredients affecting body structure/function means that an anti-wrinkle effect at 0.005% is considered unlikely to be a drug effect, but higher concentrations could potentially be classified as such. It is not listed as restricted or prohibited in the EU.


Your Skin

Yes Normal
Yes Dry
Yes Oily
Yes Sensitive
Irritancy Low
Comedogenicity Low

Our Assessment

Questionable

While Acetyl Hexapeptide-24 Amide demonstrates potent anti-aging effects by reducing muscle contractions, stimulating collagen, and boosting hydration, its practical efficacy in cosmetic products is questionable due to a significant disparity between clinically effective concentrations and the very low safety limit established by regulatory bodies, for which data is sufficient.


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